Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
STEMCELL Technologies RoboSep™ Buffer (5X Concentrate), Size: 250 mL
RoboSep™ Buffer is recommended for EasySep™ cell separation protocols performed by RoboSep™ . Please note that one or two bottles of buffer are included with every purchase of a RoboSep™ Reagent Kit.
Hoefer, Inc MOPS-SDS RUNNING BUFFER 1LX10
Mighty Small precast gels Hoefer Mighty Small precast gels are high-performance mini polyacrylamide gels commonly used for protein separation.Utilizing the Bis-Tris buffer system, they effectively minimize protein modifications during electrophoresis, resulting in sharper and clearer protein bands with higher resolution. High Loading Capacity Maximum sample volume is 50 μl Good Repeatability Fully automated gel casting process, minimal batch-to-batch variation, high stability, and good repeatability High Resolution Upgraded Bis-Trissystem for clearer, sharper separation bands and higher resolution Highly Effective Maximum 40 min for electrophoresis Ready to use and easy to store No solution preparation or gel casting is required, saving time. Store at 2-8°C Safe and non-toxic No hazardous reagents need to be handled Specifications Plate Size: 10×8 cmWells: 12-well, 15-well Concentration:• Gradient gels: 4-12ĉĉĉĉĉĉĉĉĉĉMOPS-SDS Running Buffer, 1L×10ĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉ27-Mar-25 07:45:08:000 PMĉ
Hoefer, Inc MSML PRECSTGEL 10 BIS-TRIS 12W
Mighty Small precast gels Hoefer Mighty Small precast gels are high-performance mini polyacrylamide gels commonly used for protein separation.#11;Utilizing the Bis-Tris buffer system, they effectively minimize protein modifications during electrophoresis, resulting in sharper and clearer protein bands with higher resolution. High Loading Capacity Maximum sample volume is 50 μl Good Repeatability Fully automated gel casting process, minimal batch-to-batch variation, high stability, and good repeatability High Resolution Upgraded Bis-Trissystem for clearer, sharper separation bands and higher resolution Highly Effective Maximum 40 min for electrophoresis Ready to use and easy to store No solution preparation or gel casting is required, saving time. Store at 2-8°C Safe and non-toxic No hazardous reagents need to be handled Specifications Plate Size: 10×8 cm#11;Wells: 12-well, 15-well Concentration:#11;• Gradient gels: 4-12%ĉĉĉĉĉĉĉĉĉĉMighty Small precast gels, 10% Bis-Tris, 12wellsĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉ27-Mar-25 07:45:08:000 PMĉ
National Diagnostics TBE BUFFER (10X) 4 LT
TBE Buffer (10X), Formulated with 18MegOhm water. 0.2 micron filtration. The most stable TBE on the market, 4 LT
National Diagnostics TRIS-GLYCINE-SDS PAGE BUFFER (
Buffer; National Diagnostics; Tris-Glycine-SDS PAGE (10X); Formulated w/18 Megohm water and 0.2u filtration; Saves time and improves results; Size: 1L
National Diagnostics PROTOGEL RESOLVING BUFFER 1 LT
ProtoGel Resolving Buffer (4X), 4X Laemmli Tris-HCl resolving gel buffer for clear, reproducible SDS-PAGE, 1 LT
National Diagnostics TRIS-GLYCINE-SDS PAGE BUFFER (
Tris-Glycine-SDS PAGE Buffer (10X), Ultra-pure concentrated standard running buffer for SDS-PAGE. Formulated with 18 MegOhm water. 0.2 micron filtration, 4 LT
Teknova 1X PBS-MK with Sodium Chloride, 1000mL, Sterile.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Sigma Aldrich Fine Chemicals Biosciences HEPES 99.5 (titration), 7365-45-9, MFCD00006158
HEPES 99.5 (titration)
Boston Bioproducts Inc Tris Buffered Saline, TBS (10X, pH 7.4, Low Endotoxin) - 100ml
The Tris Buffered Saline (TBS) is an isotonic non-toxic buffer solution used commonly in biochemical immunoassay as a wash buffer. Tris maintains a stable pH range typically between 7.4 to 8.0 while NaCl regulates osmotic balance via its elected concentration. This TBS Solution is formulated at a 10X concentration and tested for endotoxin.
ILEX LIFE SCIENCES LLC Promed Bioscience Atelocollagen Ultrapure, Porcine Type I, Enzymatic extraction, dialyzed, lyophilized, 10 mg
Atelocollagen Ultrapure, Porcine Type I, Enzymatic Extraction, Dialyzed, Lyophilized. Characterized by rich and ultra dialysis-purified Type I collagen content in a regular fibril-like configuration that retains its superior functionality and structural properties. The proprietary pepsin enzyme-based extraction removes telopeptides at the nonhelical N and C termini, resulting in a triple helix configuration that exhibits reduced immunogenicity. APPLICATION: Biocompatible and biodegradable, suitable for many research applications, such as bioink for 3D bioprinting, in vitro cell culture, cell functional assays, etc. MANUFACTURER: Promed Bioscience Ltd. CAS NO.: 9007-34-5. PURITY: greater than 97%. SOURCE: Porcine tendon. AMINO ACID ANALYSIS: Typical profile. STERILITY TESTING: Pass (0 cfu/g). STERILIZATION METHOD: Produced under strict aseptic conditions. CELL ATTACHMENT ASSAY: Pass. CELL TOXICITY ASSAY: Pass. OTHER NAMES: Type 1 Collagen, Collagen-1.
ILEX LIFE SCIENCES LLC GELATIN SOLUTION 0.1 500ML
PAN-Biotech Gelatin Solution 0.1% in DPBS, w/o: Ca, Mg (500 ml). DESCRIPTION: PAN-Biotech Gelatin Solution 0.1% in DPBS, w/o: Ca, Mg for laboratory research applications. Gelatin is a heterogenous mixture of water soluble proteins of high average molecular weight present in collagen. The proteins are extracted by boiling skin, tendons, ligaments, or bones in water. APPLICATION: Applications using gelatin include coating cell culture plates to improve cell attachment for a variety of cell types, addition to PCR to stabilize Taq DNA Polymerase, and use as a blocking agent in Western Blotting, ELISA, and immunohistochemistry. In bacteriology, gelatin can be used as a component of culture media. STORAGE: +2-8°C. MANUFACTURER: PAN-Biotech GmbH. COUNTRY OF ORIGIN: Germany.